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In Vitro Cytotoxicity Testing with Fluorescence-Based Assays in Cultured Human Lung and Dermal Cells

Identifieur interne : 002217 ( Main/Exploration ); précédent : 002216; suivant : 002218

In Vitro Cytotoxicity Testing with Fluorescence-Based Assays in Cultured Human Lung and Dermal Cells

Auteurs : A. Yang [États-Unis] ; D. L. Cardona [États-Unis] ; F. A. Barile [États-Unis]

Source :

Cell Biology and Toxicology [ 0742-2091 ] ; 2002-04-01.

RBID : ISTEX:D215596C82585350CB1F34AE7D33730A8C9D074A

Descripteurs français

KwdFr :
- Cellules cancéreuses en culture, Cellules cultivées, Fluorescence, Humains, Peau (), Peau (cytologie), Poumon (), Poumon (cytologie), Techniques in vitro, Tests de toxicité.
MESH :
- cytologie : Peau, Poumon.
Pascal (Inist)
- Cellules cancéreuses en culture, Cellules cultivées, Composé chimique, Culture cellulaire, Détection, Fibroblaste, Fluorescence, Homme, Humains, In vitro, Lignée A549, Lignée HFL1, Lignée WS1, Méthode étude, Peau, Poumon, Techniques in vitro, Test cytotoxicité, Tests de toxicité, Toxicité.
Wicri :
- topic : Composé chimique, Homme.

English descriptors

KwdEn :
- Cell culture, Cells, Cultured, Chemical compound, Cytotoxicity test, Detection, Fibroblast, Fluorescence, Human, Humans, In Vitro Techniques, In vitro, Investigation method, Lung (cytology), Lung (drug effects), Skin, Skin (cytology), Skin (drug effects), Sytox®, Toxicity, Toxicity Tests, Tumor Cells, Cultured, calcein-AM, cell culture, cytotoxicity, human dermal fibroblasts, human lung fibroblasts, in vitro.
MESH :
- cytology : Lung, Skin.
- drug effects : Lung, Skin.
- Cells, Cultured, Fluorescence, Humans, In Vitro Techniques, Toxicity Tests, Tumor Cells, Cultured.

Abstract

Abstract: An in vitro study using human cultured cells was conducted to determine the reliability of fluorescence-based cell viability indicators with traditional in vitro cytotoxicity testing methods. Human lung epithelial carcinoma (A549) cells, and human embryonic skin (WS1) and lung (HFL1) fibroblasts were studied in culture to evaluate their potential to screen for cytotoxicity and to compare to previous protocols conducted in our laboratory. Confluent monolayers were incubated in the absence or presence of increasing concentrations of test chemicals for 24 h, and fluorescent-labeled probes were used to assess toxicity. Eight chemicals, including mercuric chloride, copper sulfate, sodium fluoride, thioridazine HCl, paraquat, amitriptyline-HCl, verapamil-HCl and chloroquine sulfate, were tested with each cell line using calcein-AM and Sytox®. The data suggest that fluorescent probes are sensitive indicators of cytotoxicity and contribute to understanding the mechanisms for each chemical. In combination with previously published reports, the similarity of results among cell lines may be explained by the origin of the cell lines rather than by the diversity of the methods and indicators employed.

Url:

https://api.istex.fr/ark:/67375/VQC-D5LMKFKH-D/fulltext.pdf

DOI: 10.1023/A:1015328100876

Affiliations:

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<front><div type="abstract" xml:lang="en">Abstract: An in vitro study using human cultured cells was conducted to determine the reliability of fluorescence-based cell viability indicators with traditional in vitro cytotoxicity testing methods. Human lung epithelial carcinoma (A549) cells, and human embryonic skin (WS1) and lung (HFL1) fibroblasts were studied in culture to evaluate their potential to screen for cytotoxicity and to compare to previous protocols conducted in our laboratory. Confluent monolayers were incubated in the absence or presence of increasing concentrations of test chemicals for 24 h, and fluorescent-labeled probes were used to assess toxicity. Eight chemicals, including mercuric chloride, copper sulfate, sodium fluoride, thioridazine HCl, paraquat, amitriptyline-HCl, verapamil-HCl and chloroquine sulfate, were tested with each cell line using calcein-AM and Sytox®. The data suggest that fluorescent probes are sensitive indicators of cytotoxicity and contribute to understanding the mechanisms for each chemical. In combination with previously published reports, the similarity of results among cell lines may be explained by the origin of the cell lines rather than by the diversity of the methods and indicators employed.</div>
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In Vitro Cytotoxicity Testing with Fluorescence-Based Assays in Cultured Human Lung and Dermal Cells

In Vitro Cytotoxicity Testing with Fluorescence-Based Assays in Cultured Human Lung and Dermal Cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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